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Facility

Keck-II

Fourier Transform Infrared Spectroscopy (FT-IR)


FT-IR

The Nexus 870 spectrometer (Thermo Nicolet) is acquired through the financial aid of National Science Foundation (Grant No.: DMR-0216899). The Nexus 870 spectrometer is equipped with a tabletop optics module (TOM) that is used for Polarization Modulation Infrared Reflection Absorption Spectroscopy. It has an extended spectral range from 25,000 to 400cm-1 with the resolution of 0.125cm-1. The capability of Thermo Nicolet FTIR system includes:

  • Regular adsorption-transmission FTIR spectroscopy

  • Attenuated total reflection (ATR) spectroscopy

  • Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS)

  • Polarization Modulation Infrared Reflection Absorption Spectroscopy (PM-IRRAS)

  • Spectral wavelength range from 400 nm to 25,000 nm (Visible, NIR, and Mid-IR)

PM-IRRAS is commonly applied to monolayers and thin layers on metal substrates especially in molecular orientation and conformation in polymer films, biological and pharmaceutical application. For example, the PM-IRRAS at the air-water interface is used to investigate the interaction of peptides and proteins with lipid monolayers. Because only the proteins bound to or incorporated to the lipid monolayer are detected by IRRAS, this allows analysis of the change in the amide bands of proteins caused by the possible conformational variation upon binding.

The powerful software has the following capability:

  • Data collection

  • Data processing

  • Search and example library

  • Spectral interpretation guide

  • Time resolved FT-IR


More information below: