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Facility

BioCryo

Instrument details



 

Hitachi-HD2300A

BioCryo Hitachi HD-2300A Dual EDS Cryo S/TEM

  • High brightness FEG operated at 80, 120 and 200 kV.

  • Multiple STEM imaging modalities (BF, ADF, HAADF) down to ~ 0.23 nm.

  • Secondary electron detector for SE imaging.

  • Gatan diffraction CCD for nano-diffraction and diffractive imaging.

  • Ultra-sensitive dual-EDS capability.

  • Gatan Enfina EELS system.

  • Hitachi full-space tilting holder for electron tomography.

  • Gatan Cryo-TEM holder and transfer system.

  • Location: Silverman Hall, B541.

  • Hitachi HD-2300A Manual



   
HT-7700

BioCryo Hitachi HT-7700 Biological S/TEM

  • LaB6 or W filament operated from 40 to 120 kV at 100V increments.

  • Resolution 0.20nm lattice.

  • STEM unit at 1 nm resolution.

  • Magnification of 50-1000x, with 200x - 600,000x zoom.

  • Image rotation +/- 90˚ in 15˚ steps.

  • Bruker EDS system for elementary analysis.

  • Gatan Cryo-TEM holder and transfer system.

  • Location: Cook Hall, 01150B.
     
  • Hitachi HT7700 Manual
     

JEOL 123

  
BioCryo JEOL 1230 TEM

  • LaB6 or W filament operated from 40 to 120 kV at 100V increments.

  • Designed for conventional ultrastructure examinations as well as for cryo-TEM.

  • The 1230 is equipped with a Hamamatsu ORCA side-mounted camera, a Gatan 831 bottom-mounted CCD camera, and AMT imaging software for capturing high-resolution digital images.

  • JEOL Cryo-TEM holder and transfer system.
     
  • Location: Hogan, 5-126.


SEM S-4800

Hitachi S4800-II cFEG SEM

  • Cold source Field Emission SEM

  • Leica cryo stage with VCT100 transfer system and suite of cryogenic preparation equipment.

  • Oxford INCA EDS System.

  • Location: Cook Hall, 01120.
      
  • Hitachi S4800-II cFEG SEM Manual

 



High Pressure Freezer HPM100 (Leica)

  • high pressure freezer

    High-pressure freezing is the method of choice for cryofixation of pristine samples with thickness of up to 400 µm without chemical fixation and drying steps.

  • The formation and growth of ice crystals is suppressed by applying 2100 bar high pressure to the sample and cryo immobilization in less than 10 msec.

  • Once the sample is high pressure frozen, it can be processed for cryoSEM, cryoTEM, freeze substitution, and other applications.

  • Available is the flat carrier system and the tube system.

  • Location: Hogan, 5-150.



vitrobot Biocryo FEI Vitrobot Mark IV and Mark III- Plunge Freezer

  • Fully automated vitrification robot for plunge-freezing of aqueous sample suspensions.

  • All essential vitrification parameters, such as temperature, relative humidity, the pressure and quantity of blottings, and drain time can be programmed for each individual application and set for automatic retrieval.

  • Operating parameters: Working temperature 4 – 60°C (at an ambient temperature range between 18 – 25°C) Peltier controlled heating/cooling Relative humidity ambient humidity – 100% (no condensation at an RH < 85%) Ultrasonic controlled humidification.

  • Location: the Mark IV is in Hogan 5-150, the Mark III is in Cook Hall, 01152A.

  • Vitrobot Manual


Leica EM AFS2 Auto Freeze Substitution
  

Biocryo Leica EM AFS2 Automatic Freeze Substitution and Low Temperature Embedding System

  • Freeze substitution and progressive lowering of temperature (PLT) techniques along with allowing low temperature embedding and polymerization of resins.

  • LED illumination for easy viewing and working within the chamber.

  • The automatic reagent handling system EM FSP dispenses and automatically dilutes reagents from 100% stock containers.

  • “Deep Freeze” mode allows sample transfer at temperatures below -140°C

  • Fume exhaust system for operator safety

  • Efficient UV polymerization with built-in LED UV lamp.

  • Up to 20 samples can be processed automatically per run.

  • Location: Hogan, 5-150.


Leica UC7/FC7 Cryo-Ultramicrotome

Leica UC7/FC7 Cryo-Ultramicrotome

  • The Leica UC7/FC7 is a state-of-the art Cryo-Ultramicrotome capable of sectioning samples at ambient temperature down to temperatures as low as -180 C.

  • The FC7 cryo attachment is equipped with an anti-static device and a micromanipulator to assist the user to transfer delicate cryogenic thin sections to the EM grid.

  • We use diamond knives, or glass knives fabricated with our Leica KM3 Knife Maker for sectioning.

  •  It can be used for sectioning samples at liquid nitrogen temperatures, e.g. high-pressure frozen biological specimens or soft matter samples, e.g. polymers and hydrogels, for imaging with cryo-TEM and cruo-SEM.

  • The thickness of sections can be adjusted from a few nanometers to up to 15 micrometers.

  • Superior optics and illumination assist in challenging ultramicrotomy techniques such as serial sectioning or en face sectioning of cell monolayers.

  • Location: Silverman Hall, B545.
       
  • Leica UC7/FC7 Cryo-Ultramicrotome Manual
          

Leica ACE

  
Leica EM ACE600 High Vacuum Coater

  • A high vacuum film deposition system for cryo-SEM and cryo S/TEM applications.

  • It is used for freeze-fracture and sublimation of ice (etching) at controlled temperatures.

  • Provides very fine coating of frozen samples at high vacuum (10-6 mbar range) with e-beam metal/carbon (Pt/C), and with carbon evaporation.

  •  Location: Cook Hall, 01152A.
     

     


Pelco BioWave Microwave
  

PELCO BioWave Microwave Tissue Processor

  • Provides highest quality specimen preparation.

  • It accelerates sample processing: specimens can be resin embedded in one-tenth the normal time, with significantly less extraction.

  • Location: Hogan 5-150.

     


BioCryo Tousimis Critical Point Dryer

 

BioCryo Tousimis Critical Point Dryer ( Samdri-795)

  • This CPD is the standard device for critical point drying biologica; and soft matter samples (e.g. cells, tissues, hydrogels) for SEM and STEM.

  • By replacing solvents with liquid CO2 and then drying the sample above the critical point of CO2, phase boundaries between the liquid- and gaseous phases of CO2 are omitted

  • The samples dry without structural damages caused by boundary forces between different phases.

  • Location: Cook Hall, 01112.

  • Tousimis Critical point Dryer Manual

 

BioCryo VT1200 S Vibrating-blade Microtome ( "Vibratome")

Vibratome

  • The VT1200 S is a fully automatic microtome with vibrating blade.

  • It can be operated in automatic as well as in semiautomatic sectioning mode.

  • The specimen size can be up to 30 x 40 mm (in automatic mode).

  • The section thickness can be set in 1 µm increments to maximally 1000 µm (=1mm).

  • Several other parameters can be adjusted: sectioning speed, knife amplitude and cutting range.

  • The buffer chamber can be cooled with the attached chiller.

  • Ideal for making thin slices of soft samples (tissues, hydrogels, etc.) before further processing (e.g. high-pressure freezing, plunge freezing, immunolabeling).



   

BioCryo Cressington 208HR Sputter Coater

Cressington

  • The Cressington 208HR Sputter Coater system is equipped with separate rotary, planetary and tilting stage movements to ensure uniform coating.

  • The MTM-20 High Resolution Thickness Controller enables precise thickness settings for a wide choice of sputtering material. 

  • Current targets include Au, Au/Pd, Pt/Pd, Ti, and Chromium.