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Instrument details

JEOL 3200FS TEMJeol 3200FS TEM

  • FEG (Schottky)
  • 300 kV acceleration voltage

  • 0.22 nm TEM resolution

  • In-column energy filter

  • Gatan K2 Summit direct detector

  • Gatan Orius SC200 CCD

  • Gatan ELSA cryo transfer holder and JEOL RT sample holders

  • Location: Tech AB, room AG 78

  • JEOL 3200FS manual is available in NUCore under the Docs tab


 JEOL 1400 Flash TEMJEOL Flash 1400

  • LaB6 filament

  • 120 kV down to 40kV acceleration voltage

  • High resolution (HR) mode imaging with 0.27 nm (lattice) at 120 kV, 0.44 nm at 60 kV, and 0.82 nm at 40 kV

  • Suitable for RT imaging and for cryo-TEM of plunge frozen samples

  • Gatan cryo transfer holders for cryo-TEM.

  • Gatan OneView camera

  • Location: Silverman Hall B530A

  • JEOL 1400 Flash manual available in NUCore under the Docs tab




Hitachi HD-2300A Dual EDS Cryo S/TEM

  • High brightness FEG operated at 80, 120 and 200 kV.

  • Multiple STEM imaging modalities (BF, ADF, HAADF) down to ~ 0.23 nm.

  • Secondary electron detector for SE imaging.

  • Gatan diffraction CCD for nano-diffraction and diffractive imaging.

  • Ultra-sensitive dual-EDS capability.

  • Gatan Enfina EELS system.

  • Hitachi full-space tilting holder for electron tomography.

  • Gatan Cryo-TEM holder and transfer system.

  • Location: Silverman Hall, B541.

  • Hitachi HD-2300A Manual is available in NUCore under Docs tab

JEOL 1230

 JEOL 1230 TEM

  • LaB6 or W filament operated at 120 kV.

  • Designed for conventional ultrastructure examinations as well as for cryo-TEM.

  • The 1230 is equipped with a Hamamatsu ORCA side-mounted camera and a Gatan Orius 831 bottom-mounted CCD camera.

  • Gatan Cryo-TEM holder and transfer system.
  • Location: Hogan, 5-126.

SEM S-4800

Hitachi S4800-II cryo SEM

  • Cold source Field Emission SEM

  • Leica cryo stage with VCT100 transfer system and suite of cryogenic preparation equipment.

  • Oxford INCA EDS System.

  • Location: Technology Institute, AG 83.
  • For cryo-SEM contact BioCryo
  • Manual for the Hitachi S4800-II is available in NUCore in the Docs tab



Lecia High Pressure Freezer HPM100 (Leica)

  • high pressure freezer

    High-pressure freezing is the method of choice for cryofixation of pristine samples with thickness of up to 400 µm without chemical fixation and drying steps.

  • The formation and growth of ice crystals is suppressed by applying 2100 bar high pressure to the sample and cryo immobilization in less than 10 msec.

  • Once the sample is high pressure frozen, it can be processed for cryoSEM, cryoTEM, freeze substitution, and other applications.

  • Available is the flat carrier system and the tube system.

  • Location: Hogan, 5-150.

vitrobot  FEI Vitrobot Mark IV and Mark III- Plunge Freezer

  • Fully automated vitrification robot for plunge-freezing of aqueous sample suspensions.

  • All essential vitrification parameters, such as temperature, relative humidity, the pressure and quantity of blottings, and drain time can be programmed for each individual application and set for automatic retrieval.

  • Operating parameters: Working temperature 4 – 60°C (at an ambient temperature range between 18 – 25°C) Peltier controlled heating/cooling Relative humidity ambient humidity – 100% (no condensation at an RH < 85%) Ultrasonic controlled humidification.

  • Location: the Mark IV is in Hogan 5-150, the Mark III is in Technology Institute, AG 76

  • Vitrobot Manual is avaiable in NUCore in Docs tab

Leica EM AFS2 Auto Freeze Substitution

Leica EM AFS2 Automatic Freeze Substitution and Low Temperature Embedding System

  • Freeze substitution and progressive lowering of temperature (PLT) techniques along with allowing low temperature embedding and polymerization of resins.

  • LED illumination for easy viewing and working within the chamber.

  • The automatic reagent handling system EM FSP dispenses and automatically dilutes reagents from 100% stock containers.

  • “Deep Freeze” mode allows sample transfer at temperatures below -140°C

  • Fume exhaust system for operator safety

  • Efficient UV polymerization with built-in LED UV lamp.

  • Up to 20 samples can be processed automatically per run.

  • Location: Hogan, 5-150.

Leica UC7/FC7 Cryo-Ultramicrotome

Leica UC7/FC7 Cryo-Ultramicrotome

  • The Leica UC7/FC7 is a state-of-the art Cryo-Ultramicrotome capable of sectioning samples at ambient temperature down to temperatures as low as -180 C.

  • The FC7 cryo attachment is equipped with an anti-static device and a micromanipulator to assist the user to transfer delicate cryogenic thin sections to the EM grid.

  • We use diamond knives, or glass knives fabricated with our Leica KM3 Knife Maker for sectioning.

  •  It can be used for sectioning samples at liquid nitrogen temperatures, e.g. high-pressure frozen biological specimens or soft matter samples, e.g. polymers and hydrogels, for imaging with cryo-TEM and cruo-SEM.

  • The thickness of sections can be adjusted from a few nanometers to up to 15 micrometers.

  • Superior optics and illumination assist in challenging ultramicrotomy techniques such as serial sectioning or en face sectioning of cell monolayers.

  • Location: Silverman Hall, B545.
  • Leica UC7/FC7 Cryo-Ultramicrotome Manual is available in NUCore in Docs tab

Leica ACE

Leica EM ACE600 High Vacuum Coater

  • A high vacuum film deposition system for cryo-SEM and cryo S/TEM applications.

  • It is used for freeze-fracture and sublimation of ice (etching) at controlled temperatures.

  • Provides very fine coating of frozen samples at high vacuum (10-6 mbar range) with e-beam metal/carbon (Pt/C), and with carbon evaporation.

  •  Location: Technology Institute, AG 76


Pelco BioWave Microwave

PELCO BioWave Microwave Tissue Processor

  • Provides highest quality specimen preparation.

  • It accelerates sample processing: specimens can be resin embedded in one-tenth the normal time, with significantly less extraction.

  • The manual for the PELCO Processor is available in NUCore in Docs tab
  • Location: Hogan 5-150.


BioCryo Tousimis Critical Point Dryer


 Tousimis Critical Point Dryer (Samdri-795)

  • This CPD is the standard device for critical point drying biologica; and soft matter samples (e.g. cells, tissues, hydrogels) for SEM and STEM.

  • By replacing solvents with liquid CO2 and then drying the sample above the critical point of CO2, phase boundaries between the liquid- and gaseous phases of CO2 are omitted

  • The samples dry without structural damages caused by boundary forces between different phases.

  • Location: Hogan Hall 5-150

  • Tousimis Critical point Dryer Manual is available in NUCore in Docs tab


 Lecia VT1200 S Vibrating-blade Microtome ( "Vibratome")


  • The VT1200 S is a fully automatic microtome with vibrating blade.

  • It can be operated in automatic as well as in semiautomatic sectioning mode.

  • The specimen size can be up to 30 x 40 mm (in automatic mode).

  • The section thickness can be set in 1 µm increments to maximally 1000 µm (=1mm).

  • Several other parameters can be adjusted: sectioning speed, knife amplitude and cutting range.

  • The buffer chamber can be cooled with the attached chiller.

  • Ideal for making thin slices of soft samples (tissues, hydrogels, etc.) before further processing (e.g. high-pressure freezing, plunge freezing, immunolabeling).

  • Manual for the VT1200 is available in NUCore in Docs tab 
  • Location: Hogan Hall 5-155



ASP-1000 mPrep Automated Specimen Processor

  • The ASP-1000 automates the full range of electron microscopy sample preparation; fully programmable  uses both mPrep/s and mPrep/g capsules to perform an unlimited range of lab preparation tasks, including:

  • TEM specimen preparation (resin embedment)

  • TEM grid staining

  • Immuno-labeling of TEM grids

  • Immuno-labeling of en bloc tissue